Identification of a response regulator involved in surface attachment, cell–cell aggregation, exopolysaccharide production and virulence in the plant pathogen Xylella fastidiosa
Identifieur interne : 002138 ( Main/Exploration ); précédent : 002137; suivant : 002139Identification of a response regulator involved in surface attachment, cell–cell aggregation, exopolysaccharide production and virulence in the plant pathogen Xylella fastidiosa
Auteurs : Tanja M. Voegel [États-Unis, Canada] ; Harshavardhan Doddapaneni [États-Unis] ; Davis W. Cheng [États-Unis] ; Hong Lin [États-Unis] ; Drake C. Stenger [États-Unis] ; Bruce C. Kirkpatrick [États-Unis] ; M. Caroline Roper [États-Unis]Source :
- Molecular Plant Pathology [ 1464-6722 ] ; 2013-04.
English descriptors
- Teeft :
- Adhesins, Agar medium, Aggregated cells, Aggregation, Amino acids, Annu, Assay, Bacterial genomes, Blackwell publishing, Bspp, Causal agent, Cdna, Cell aggregates, Cell growth, Complete list, Difference test, Error bars, Exopolysaccharide, Fastidiosa, Fastidiosa cells, Fastidiosa cultures, Fastidiosa genome, Fastidiosa matrix, Fems microbiol, Fetzer, Functional groups, Gene, Gene expression, Gene regulation, Genome, Grapevines inoculated, Guilhabert, Hydrogen peroxide, Immunosorbent assay, Inoculated, Iron uptake, Kirkpatrick, Life technologies, Liquid medium, Matrix production, Microarray, Microarray analysis, Microarray data, Microbiol, Molecular plant pathology, Mutant, Mutant strain, Oligos, Optical density, Other bacteria, Output domains, Pathway, Phenotype, Phosphoryl group, Plant pathology, Plants inoculated, Potential virulence factors, Primer, Primer pair, Purcell, Receiver domain, Regulator, Regulatory systems, Roper, Sensor kinase, Signal transduction, Silva, Solid surface, Standard curve, Standard deviation, Surface attachment, Survival rate, Tcst, Time point, Transcriptional, Transduction, Trends biochem, Virulence, Virulence factors, Vitis vinifera, Voegel, Xanthomonas oryzae, Xhpt, Xylella, Xylella fastidiosa, Xylella xhpt response regulator, Xylem vessels.
Abstract
Xylella fastidiosa, the causal agent of Pierce's disease of grapevine, possesses several two‐component signal transduction systems that allow the bacterium to sense and respond to changes in its environment. Signals are perceived by sensor kinases that autophosphorylate and transfer the phosphate to response regulators (RRs), which direct an output response, usually by acting as transcriptional regulators. In the X. fastidiosa genome, 19 RRs were found. A site‐directed knockout mutant in one unusual RR, designated XhpT, composed of a receiver domain and a histidine phosphotransferase output domain, was constructed. The resulting mutant strain was analysed for changes in phenotypic traits related to biofilm formation and gene expression using microarray analysis. We found that the xhpT mutant was altered in surface attachment, cell–cell aggregation, exopolysaccharide (EPS) production and virulence in grapevine. In addition, this mutant had an altered transcriptional profile when compared with wild‐type X. fastidiosa in genes for several biofilm‐related traits, such as EPS production and haemagglutinin adhesins.
Url:
DOI: 10.1111/mpp.12004
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001C21
- to stream Istex, to step Curation: 001C21
- to stream Istex, to step Checkpoint: 000276
- to stream Main, to step Merge: 002162
- to stream Main, to step Curation: 002138
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of a response regulator involved in surface attachment, cell–cell aggregation, exopolysaccharide production and virulence in the plant pathogen Xylella fastidiosa</title><author><name sortKey="Voegel, Tanja M" sort="Voegel, Tanja M" uniqKey="Voegel T" first="Tanja M." last="Voegel">Tanja M. Voegel</name></author><author><name sortKey="Doddapaneni, Harshavardhan" sort="Doddapaneni, Harshavardhan" uniqKey="Doddapaneni H" first="Harshavardhan" last="Doddapaneni">Harshavardhan Doddapaneni</name></author><author><name sortKey="Cheng, Davis W" sort="Cheng, Davis W" uniqKey="Cheng D" first="Davis W." last="Cheng">Davis W. Cheng</name></author><author><name sortKey="Lin, Hong" sort="Lin, Hong" uniqKey="Lin H" first="Hong" last="Lin">Hong Lin</name></author><author><name sortKey="Stenger, Drake C" sort="Stenger, Drake C" uniqKey="Stenger D" first="Drake C." last="Stenger">Drake C. Stenger</name></author><author><name sortKey="Kirkpatrick, Bruce C" sort="Kirkpatrick, Bruce C" uniqKey="Kirkpatrick B" first="Bruce C." last="Kirkpatrick">Bruce C. Kirkpatrick</name></author><author><name sortKey="Roper, M Caroline" sort="Roper, M Caroline" uniqKey="Roper M" first="M. Caroline" last="Roper">M. Caroline Roper</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CFE276ECF1B32F0611A697BC7F99D283DABFF1FD</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/mpp.12004</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-48J34GZJ-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001C21</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001C21</idno><idno type="wicri:Area/Istex/Curation">001C21</idno><idno type="wicri:Area/Istex/Checkpoint">000276</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000276</idno><idno type="wicri:doubleKey">1464-6722:2013:Voegel T:identification:of:a</idno><idno type="wicri:Area/Main/Merge">002162</idno><idno type="wicri:Area/Main/Curation">002138</idno><idno type="wicri:Area/Main/Exploration">002138</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Identification of a response regulator involved in surface attachment, cell–cell aggregation, exopolysaccharide production and virulence in the plant pathogen Xylella fastidiosa</title><author><name sortKey="Voegel, Tanja M" sort="Voegel, Tanja M" uniqKey="Voegel T" first="Tanja M." last="Voegel">Tanja M. Voegel</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of California, CA, 95616, Davis</wicri:regionArea><wicri:noRegion>Davis</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Current Address: Department of Biology, University of British Columbia Okanagan, BC, Kelowna</wicri:regionArea><wicri:noRegion>Kelowna</wicri:noRegion></affiliation></author><author><name sortKey="Doddapaneni, Harshavardhan" sort="Doddapaneni, Harshavardhan" uniqKey="Doddapaneni H" first="Harshavardhan" last="Doddapaneni">Harshavardhan Doddapaneni</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Crop Diseases, Pests & Genetics, USDA‐ARS, CA, 93648, Parlier</wicri:regionArea><wicri:noRegion>Parlier</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Davis W" sort="Cheng, Davis W" uniqKey="Cheng D" first="Davis W." last="Cheng">Davis W. Cheng</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Crop Diseases, Pests & Genetics, USDA‐ARS, CA, 93648, Parlier</wicri:regionArea><wicri:noRegion>Parlier</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Hong" sort="Lin, Hong" uniqKey="Lin H" first="Hong" last="Lin">Hong Lin</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Crop Diseases, Pests & Genetics, USDA‐ARS, CA, 93648, Parlier</wicri:regionArea><wicri:noRegion>Parlier</wicri:noRegion></affiliation></author><author><name sortKey="Stenger, Drake C" sort="Stenger, Drake C" uniqKey="Stenger D" first="Drake C." last="Stenger">Drake C. Stenger</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Crop Diseases, Pests & Genetics, USDA‐ARS, CA, 93648, Parlier</wicri:regionArea><wicri:noRegion>Parlier</wicri:noRegion></affiliation></author><author><name sortKey="Kirkpatrick, Bruce C" sort="Kirkpatrick, Bruce C" uniqKey="Kirkpatrick B" first="Bruce C." last="Kirkpatrick">Bruce C. Kirkpatrick</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of California, CA, 95616, Davis</wicri:regionArea><wicri:noRegion>Davis</wicri:noRegion></affiliation></author><author><name sortKey="Roper, M Caroline" sort="Roper, M Caroline" uniqKey="Roper M" first="M. Caroline" last="Roper">M. Caroline Roper</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology and Microbiology, University of California, CA, 92521, Riverside</wicri:regionArea><wicri:noRegion>Riverside</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Molecular Plant Pathology</title><title level="j" type="alt">MOLECULAR PLANT PATHOLOGY</title><idno type="ISSN">1464-6722</idno><idno type="eISSN">1364-3703</idno><imprint><biblScope unit="vol">14</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="256">256</biblScope><biblScope unit="page" to="264">264</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2013-04">2013-04</date></imprint><idno type="ISSN">1464-6722</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1464-6722</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adhesins</term><term>Agar medium</term><term>Aggregated cells</term><term>Aggregation</term><term>Amino acids</term><term>Annu</term><term>Assay</term><term>Bacterial genomes</term><term>Blackwell publishing</term><term>Bspp</term><term>Causal agent</term><term>Cdna</term><term>Cell aggregates</term><term>Cell growth</term><term>Complete list</term><term>Difference test</term><term>Error bars</term><term>Exopolysaccharide</term><term>Fastidiosa</term><term>Fastidiosa cells</term><term>Fastidiosa cultures</term><term>Fastidiosa genome</term><term>Fastidiosa matrix</term><term>Fems microbiol</term><term>Fetzer</term><term>Functional groups</term><term>Gene</term><term>Gene expression</term><term>Gene regulation</term><term>Genome</term><term>Grapevines inoculated</term><term>Guilhabert</term><term>Hydrogen peroxide</term><term>Immunosorbent assay</term><term>Inoculated</term><term>Iron uptake</term><term>Kirkpatrick</term><term>Life technologies</term><term>Liquid medium</term><term>Matrix production</term><term>Microarray</term><term>Microarray analysis</term><term>Microarray data</term><term>Microbiol</term><term>Molecular plant pathology</term><term>Mutant</term><term>Mutant strain</term><term>Oligos</term><term>Optical density</term><term>Other bacteria</term><term>Output domains</term><term>Pathway</term><term>Phenotype</term><term>Phosphoryl group</term><term>Plant pathology</term><term>Plants inoculated</term><term>Potential virulence factors</term><term>Primer</term><term>Primer pair</term><term>Purcell</term><term>Receiver domain</term><term>Regulator</term><term>Regulatory systems</term><term>Roper</term><term>Sensor kinase</term><term>Signal transduction</term><term>Silva</term><term>Solid surface</term><term>Standard curve</term><term>Standard deviation</term><term>Surface attachment</term><term>Survival rate</term><term>Tcst</term><term>Time point</term><term>Transcriptional</term><term>Transduction</term><term>Trends biochem</term><term>Virulence</term><term>Virulence factors</term><term>Vitis vinifera</term><term>Voegel</term><term>Xanthomonas oryzae</term><term>Xhpt</term><term>Xylella</term><term>Xylella fastidiosa</term><term>Xylella xhpt response regulator</term><term>Xylem vessels</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Xylella fastidiosa, the causal agent of Pierce's disease of grapevine, possesses several two‐component signal transduction systems that allow the bacterium to sense and respond to changes in its environment. Signals are perceived by sensor kinases that autophosphorylate and transfer the phosphate to response regulators (RRs), which direct an output response, usually by acting as transcriptional regulators. In the X. fastidiosa genome, 19 RRs were found. A site‐directed knockout mutant in one unusual RR, designated XhpT, composed of a receiver domain and a histidine phosphotransferase output domain, was constructed. The resulting mutant strain was analysed for changes in phenotypic traits related to biofilm formation and gene expression using microarray analysis. We found that the xhpT mutant was altered in surface attachment, cell–cell aggregation, exopolysaccharide (EPS) production and virulence in grapevine. In addition, this mutant had an altered transcriptional profile when compared with wild‐type X. fastidiosa in genes for several biofilm‐related traits, such as EPS production and haemagglutinin adhesins.</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Voegel, Tanja M" sort="Voegel, Tanja M" uniqKey="Voegel T" first="Tanja M." last="Voegel">Tanja M. Voegel</name></noRegion><name sortKey="Cheng, Davis W" sort="Cheng, Davis W" uniqKey="Cheng D" first="Davis W." last="Cheng">Davis W. Cheng</name><name sortKey="Doddapaneni, Harshavardhan" sort="Doddapaneni, Harshavardhan" uniqKey="Doddapaneni H" first="Harshavardhan" last="Doddapaneni">Harshavardhan Doddapaneni</name><name sortKey="Kirkpatrick, Bruce C" sort="Kirkpatrick, Bruce C" uniqKey="Kirkpatrick B" first="Bruce C." last="Kirkpatrick">Bruce C. Kirkpatrick</name><name sortKey="Lin, Hong" sort="Lin, Hong" uniqKey="Lin H" first="Hong" last="Lin">Hong Lin</name><name sortKey="Roper, M Caroline" sort="Roper, M Caroline" uniqKey="Roper M" first="M. Caroline" last="Roper">M. Caroline Roper</name><name sortKey="Roper, M Caroline" sort="Roper, M Caroline" uniqKey="Roper M" first="M. Caroline" last="Roper">M. Caroline Roper</name><name sortKey="Stenger, Drake C" sort="Stenger, Drake C" uniqKey="Stenger D" first="Drake C." last="Stenger">Drake C. Stenger</name></country><country name="Canada"><noRegion><name sortKey="Voegel, Tanja M" sort="Voegel, Tanja M" uniqKey="Voegel T" first="Tanja M." last="Voegel">Tanja M. Voegel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002138 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002138 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:CFE276ECF1B32F0611A697BC7F99D283DABFF1FD
|texte= Identification of a response regulator involved in surface attachment, cell–cell aggregation, exopolysaccharide production and virulence in the plant pathogen Xylella fastidiosa
}}
| This area was generated with Dilib version V0.6.33. |  |